Manipulating Natural Product Biosynthetic Pathways via DNA Assembler
نویسندگان
چکیده
منابع مشابه
Manipulating natural product biosynthetic pathways via DNA assembler.
DNA assembler is an efficient synthetic biology method for constructing and manipulating biochemical pathways. The rapidly increasing number of sequenced genomes provides a rich source for discovery of gene clusters involved in synthesizing new natural products. However, both discovery and economical production are hampered by our limited knowledge in manipulating most organisms and the corresp...
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We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unpre...
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We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unpre...
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ژورنال
عنوان ژورنال: Current Protocols in Chemical Biology
سال: 2014
ISSN: 2160-4762,2160-4762
DOI: 10.1002/9780470559277.ch130191